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Restriction Endonucleases
Blunt- and sticky end cleaving restriction endonucleases acting on dsDNA.

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  • AccI  
    Reaction temperature: 37°C ; recognition sequence: GT | MKAC ; Prototype: AccI

  • AcvI  
    Reaction temperature: 37°C ; recognition sequence: CAC | GTG ; Prototype: PmaCI

  • AluI  
    Reaction temperature: 37°C ; recognition sequence: AG | CT ; Prototype: AluI

  • ApaI  
    Reaction temperature: 25°C ; recognition sequence: GGGCC | C ; Prototype: ApaI

  • AvaI  
    Reaction temperature: 37°C ; recognition sequence: C | YCGRG ; Prototype: AvaI

  • AvaII  
    Reaction temperature: 37°C ; recognition sequence: G | GWCC ; Prototype: AvaII

  • BalI  
    Reaction temperature: 37°C ; recognition sequence: TGG | CCA ; Prototype: BalI

  • BamHI  
    Reaction temperature: 37°C ; recognition sequence: G | GATCC ; Prototype: BamHI

  • BanII  
    Reaction temperature: 37°C ; recognition sequence: GRGCY | C ; Prototype: HgiJII

  • BglI  
    Reaction temperature: 37°C ; recognition sequence: GCCNNNN | NGGC ; Prototype: BglI

  • BglII  
    Reaction temperature: 37°C ; recognition sequence: A | GATCT ; Prototype: BglII

  • BsiHKCI  
    Reaction temperature: 65°C ; recognition sequence: C | YCGRG ; Prototype: AvaI

  • BssHII  
    Reaction temperature: 50°C ; recognition sequence: G | CGCGC ; Prototype: BsePI

  • BstXI  
    Reaction temperature: 50°C ; recognition sequence: CCANNNNN | NTGG ; Prototype: BstXI


  • ClaI  
    Reaction temperature: 37°C ; recognition sequence: AT | CGAT ; Prototype: ClaI

  • CviJI  
    Reaction temperature: 37°C ; recognition sequence: RG | CY ; Prototype: CviJI

  • CviJI*  
    Reaction temperature: 37°C ; recognition sequence: G | C except YG / CR ; Prototype: CviJI*

  • DdeI  
    Reaction temperature: 37°C ; recognition sequence: C | TNAG ; Prototype: DdeI

  • DpnI  
    Reaction temperature: 37°C ; recognition sequence: GA | TC ; Prototype: DpnI

  • DraI  
    Reaction temperature: 37°C ; recognition sequence: TTT | AAA ; Prototype: AhaI

  • EcoRI  
    Reaction temperature: 37°C ; recognition sequence: G | AATTC ; Prototype: EcoRI

  • EcoRV  
    Reaction temperature: 37°C ; recognition sequence: GAT | ATC ; Prototype: EcoRV

  • FokI  
    Reaction temperature: 37°C ; recognition sequence: GGATG (9/13) ; Prototype: FokI

  • HaeIII  
    Reaction temperature: 37°C ; recognition sequence: GG | CC ; Prototype: HaeIII

  • HhaI  
    Reaction temperature: 37°C ; recognition sequence: GCG | C ; Prototype: HhaI

  • HincII  
    Reaction temperature: 37°C ; recognition sequence: GTY | RAC ; Prototype: HindII

  • HindIII  
    Reaction temperature: 37°C ; recognition sequence: A | AGCTT ; Prototype: HindIII

  • HinfI  
    Reaction temperature: 37°C ; recognition sequence: G | ANTC ; Prototype: HinfI

  • HpaI  
    Reaction temperature: 37°C ; recognition sequence: GTT | AAC ; Prototype: HpaI

  • HpaII  
    Reaction temperature: 37°C ; recognition sequence: C | CGG ; Prototype: HpaII

  • KpnI  
    Reaction temperature: 37°C ; recognition sequence: GGTAC | C ; Prototype: KpnI

  • MboI  
    Reaction temperature: 37°C ; recognition sequence: | GATC ; Prototype: MboI

  • MboII  
    Reaction temperature: 37°C ; recognition sequence: GAAGA (8/7) ; Prototype: MboII

  • MluI  
    Reaction temperature: 37°C ; recognition sequence: A | CGCGT ; Prototype: MluI

  • MmeI  
    Discontinued. Reaction temperature: 37°C ; recognition sequence: TCCRAC (20/18) ; Prototype: MmeI

  • MnlI  
    Reaction temperature: 37°C ; recognition sequence: CCTC (7/6) ; Prototype: MnlI

  • MspI  
    Reaction temperature: 37°C ; recognition sequence: C | CGG ; Prototype: HpaII

  • NarI  
    Reaction temperature: 37°C ; recognition sequence: GG | CGCC ; Prototype: NarI

  • NcoI  
    Reaction temperature: 37°C ; recognition sequence: C | CATGG ; Prototype: NcoI

  • NdeI  
    Reaction temperature: 37°C ; recognition sequence: CA | TATG ; Prototype: NdeI

  • NheI  
    Reaction temperature: 37°C ; recognition sequence: G | CTAGC ; Prototype: NheI

  • NotI  
    Reaction temperature: 37°C ; recognition sequence: GC | GGCCGC ; Prototype: NotI

  • NruI  
    Reaction temperature: 37°C ; recognition sequence: TCG | CGA ; Prototype: NruI

  • NsiI  
    Reaction temperature: 37°C ; recognition sequence: ATGCA | T ; Prototype: NsiI

  • PinAI  
    Reaction temperature: 37°C ; recognition sequence: A | CCGGT ; Prototype: AgeI

  • PstI  
    Reaction temperature: 37°C ; recognition sequence: CTGCA | G ; Prototype: PstI

  • PvuI  
    Reaction temperature: 37°C ; recognition sequence: CGAT | CG ; Prototype: PvuI

  • PvuII  
    Reaction temperature: 37°C ; recognition sequence: CAG | CTG ; Prototype: PvuII

  • RsaI  
    Reaction temperature: 37°C ; recognition sequence: GT | AC ; Prototype: RsaI

  • RsrII  
    Reaction temperature: 37°C ; recognition sequence: CG | GWCCG ; Prototype: RsrII

  • SacI  
    Reaction temperature: 37°C ; recognition sequence: GAGCT | C ; Prototype: SacI

  • SacII  
    Reaction temperature: 37°C ; recognition sequence: CCGC | GG ; Prototype: SacII

  • SalI  
    Reaction temperature: 37°C ; recognition sequence: G | TCGAC ; Prototype: SalI

  • Sau3AI  
    Reaction temperature: 37°C ; recognition sequence: | GATC ; Prototype: MboI

  • ScaI  
    Reaction temperature: 37°C ; recognition sequence: AGT | ACT ; Prototype: ScaI

  • SfiI  
    Reaction temperature: 50°C ; recognition sequence: GGCCNNNN | NGGCC ; Prototype: SfiI


  • SmaI  
    Reaction temperature: 25°C ; recognition sequence: CCC | GGG ; Prototype: SmaI

  • SpeI  
    Reaction temperature: 37°C ; recognition sequence: A | CTAGT ; Prototype: SpeI

  • SphI  
    Reaction temperature: 37°C ; recognition sequence: GCATG | C ; Prototype: SphI

  • SspI  
    Reaction temperature: 37°C ; recognition sequence: AAT | ATT ; Prototype: SspI

  • StuI  
    Reaction temperature: 37°C ; recognition sequence: AGG | CCT ; Prototype: StuI

  • TaqI  
    Reaction temperature: 65°C ; recognition sequence: T | CGA ; Prototype: TaqI

  • TaqII  
    Reaction temperature: 65°C ; recognition sequence: GACCGA (11/9) ; Prototype: TaqII

  • TspDTI  
    Reaction temperature: 70°C ; recognition sequence: ATGAA (11/9) ; Prototype: TspDTI

  • TspGWI  
    Reaction temperature: 70°C ; recognition sequence: ACGGA (11/9) ; Prototype: TspGWI

  • Tth111I  
    Reaction temperature: 65°C ; recognition sequence: GACN | NNGTC ; Prototype: Tth111I

  • XbaI  
    Reaction temperature: 37°C ; recognition sequence: T | CTAGA ; Prototype: XbaI

  • XhoI  
    Reaction temperature: 37°C ; recognition sequence: C | TCGAG ; Prototype: XhoI


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