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Nucleases
For application in selective nucleic acid digestion reactions.

  • DNase I – RNase free  
    Nonspecific deoxyribonuclease / endonuclease acting on ssDNA and dsDNA. For DNA-free RNA preparation and for generation of random DNA libraries.

  • Exonuclease I  
    ssDNA specific, does not digest dsDNA. For easy removal of oligonucleotides from PCR reactions prior to sequencing.

  • Exonuclease III ( E.coli )  
    dsDNA specific, digests dsDNA at nicks producing single-stranded gaps, for construction of nested unidirectional deletions.

  • Lambda Exonuclease  
    Acts specific on 5'-phosphorylated dsDNA while leaving the unphosphorylated strand intact.

  • Mung Bean Nuclease  
    Used for mapping of transcripts; digests hairpin structures during cDNA synthesis, does not cleave opposite a nick.

  • OMNI Nuclease  
    Nonspecific nuclease that completely degrades all forms of DNA and RNA (single- and double-stranded).

  • RNase I (Ribonuclease I)  
    Only available completely nonspecific ribonuclease that hydrolyzes phosphodiester bond after all four bases.

  • RNase III (Ribonuclease III)  
    Cleaves long double-stranded RNA (dsRNA) into short dsRNAs (13-30 bases), which, upon transfection, can induce RNAi.



  • S1 Nuclease  
    Non-specific endonuclease, active on ssDNA and RNA, nicks, mismatches and gaps; for S1 mapping of nucleic acids.


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