Figure: Robust short, medium and long range PCR amplifications with OptiTaq DNA Polymerase (Cat. No. E2600) in a size range between ˜1 and 20 kb. Molecular weight markers MW2 (Lambda-DNA [Phage λ, GenBank J02459] / Hind III) and MW1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 20 kb: PCR amplification reactions.
OptiTaq DNA Polymerase - Package Contents
OptiTaq DNA Polymerase
10x Buffer Pol B (Standard reaction buffer containing MgCl2)
10x Buffer Pol C (Purple coloured reaction buffer, for direct gel loading)
10x Buffer Pol A (Optimization reaction buffer without MgCl2)
25 mM MgCl2 solution
OptiTaq DNA Polymerase - Kit Package Contents
Same as OptiTaq DNA Polymerase, plus ultrapure dNTP solution