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OMNI Nuclease

Nonspecific nuclease that completely degrades all forms of DNA and RNA (single- and double-stranded).

Detailed Product Description
 English Version
 Deutsche Version

Quantity Package Cat-No. Price in €
20 000 UE1120-0133.00
100 000 UE1120-02149.00



General Description

OMNI Nuclease is a non-specific nuclease, catalyzing removal of all forms of nucleic acids (DNA and RNA; linear, circular, double and single stranded). The nuclease completely degrades nucleic acids to 5'-monophosphorate terminated oligonucleotides with a length of 2 – 6 nt.

Applications
  • Sample preparation for protein 2D electrophoresis
  • Removal of nucleic acids contaminants from protein preparations
  • Viscosity reduction in bacterial, yeast and mammalian protein extracts

Application Example


Figure 1: OMNI Nuclease activity test. Incubation of DNA (lanes 1 to 4) and RNA (lanes 5 to 8) with increasing amounts of OMNI nuclease. Digestion was performed at 37°C for 30 min. Digestion efficiency was monitored by 1% [w/v] agarose gel electrophoresis.
  • Lanes 1-4: 37 µg Herring sperm DNA, with increasing amounts of OMNI Nuclease units from lane 1 to 4.
  • Lanes 5-8: Torula Yeast RNA type VI, with increasing amounts of OMNI Nuclease units from lane 5 to 8.
  • K-: Control lanes without OMNI Nuclease addition.
  • MW: Molecular weight marker.

Quality Control / Purity Check


Figure 2: Purity check of OMNI nuclease on a 12% PAGE protein gel.
  • Left lane: Protein molecular weight marker (numbers indicate MW in kDa).
  • Right lane: 5 µg OMNI nuclease.