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Probe qPCR Master Mix


Sensitive, high precision (2x) master mix for probe-based quantitative real-time PCR and two-step RT-qPCR. Plus separate UNG and PCR water. No ROX.


Detailed product description
    English version       Deutsche Version  

Quantity Package Cat-No. Price in €
100 reactions
(100 x 25 μl)
E0420-0146.00
200 reactions
(200 x 25 μl)
E0420-0289.00
1000 reactions
(1000 x 25 μl)
E0420-03390.00




Application Example



Figure 1: Probe-based real-time PCR plus UNG pretreatment with EURx Probe qPCR Master Mix (2x), GAPDH specific primers and a HEX-labeled probe binding within the amplicon. Reactions were performed in duplicates by using human leukocyte cDNA (10 ng and 0.2 ng) as template. Measured CT values as well as amplification plots were near identical for all corresponding duplicates, respectively. For each template DNA amount, amplification plots for one of both duplicates is displayed, respectively. Measured PCR efficiency was 98%.

Probe qPCR Master Mix (2x) - Package Contents Optionally supplied, if needed:
  • 25 mM MgCl2 solution. If required, please request this component along with your order.
Related Products Additional Resources

 qPCR Master Mix Logsheet

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