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NXT Taq - Fast PCR Kit

Fast "Hot Start" Taq DNA polymerase, MAB inhibited.
  • "High throughput" genotyping, diagnostic PCRs.
  • No "proofreading".
  • Convenient, stable master mix.
  • Contains separate, optional Color Load solution for direct gel loading.

Detailed product description
    English version       Deutsche Version  

Quantity Package Cat-No. Price in €
100 reactions (20 μl)E2530-0140.00
200 reactions (20 μl)E2530-0278.00
500 reactions (20 μl)E2530-03160.00

Enables to conduct complete PCR runs within dramatically shortened amplification times.

Application Example

Figure: Fast PCR amplification using NXT Taq - FAST PCR Kit.

A 2.5 kb amplicon of the human CCR5 gene was amplified with NXT Taq PCR Kit and the following PCR conditions:

95°C 5 min – 31x (96°C 5s - 60°C 5s - 68°C 1 min 15 s) - 72°C 1 min
  • Lane M: molecular size marker- (Perfect™ 1kb DNA Ladder).
  • Lanes 1,2: PCR amplification reactions using NXT Taq - FAST PCR Kit (Cat. No.E2530).
NXT Taq -FAST PCR Kit - Package Contents
  • 1. NXT Taq PCR Master Mix (2x)
  • 2. Water, nuclease free
  • 3. 10 x Color Load
Additional Resources

 PCR MasterMix Logsheet

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