Click here to navigate   Home > Products > PCR > PCR – DNA Polymerases and PCR Master Mixes > "Hot Start" PCR - non-"proofreading" > Perpetual Taq Master Mix

Perpetual Taq Master Mix

"Hot Start" Taq DNA polymerase, MAB inhibited.
  • Fluorescent PCR methods, genotyping, diagnostic PCR.
  • No "proofreading".
  • Convenient, stable master mix.
  • Contains separate, optional Color Load solution for direct gel loading.

Detailed product description
    English version       Deutsche Version  

Quantity Package Cat-No. Price in €
500 reactions (50 μl)E2740-03290.00
200 reactions (50 μl)E2740-02145.00
100 reactions (50 μl)E2740-0175.00

Application Examples

Figure 1: Comparisonal HotStart PCR amplification of an 1.1 kb amplicon (human ccr5 gene) with Perpetual Taq DNA Polymerase (Cat. No. E2700, non-master-mix format)) and ready-to-use Perpetual Taq PCR Master Mix (Cat. No. 2740). Molecular weight marker MW (Perfect™ 1kb DNA Ladder). Each PCR reaction: 1.25 U Perpetual Taq DNA Polymerase, 0.2 mM dNTPs; 50 µl reaction volume. Lanes 2-3: EURx Perpetual Taq DNA Polymerase (non-master-mix format); 4-5: EURx Perpetual Taq PCR Master Mix (after 25 freeze-thaw cycles, to demonstrate tolerance for repeated freezing and thawing), 6-7: EURx Perpetual Taq PCR Master Mix (after 25 freeze-thaw cycles) plus optional Color Load (for direct post-PCR gel loading).

Figure 2: Sensitive HotStart PCR amplification with Perpetual Taq DNA Polymerase (Cat. No. E2700) in a size range between ~1 and 15 kb. Molecular weight marker M1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 15 kb: PCR amplification reactions (each reaction 1.25 U EURx Perpetual Taq DNA Polymerase, Pol Buffer B, 0.2 mM dNTPs; 50 µl reaction volume).

Perpetual Taq PCR Master Mix (2x) - Package Contents
  • Taq PCR Master Mix (2x)
  • PCR Water (Nuclease Free)
  • 10x Color Load (Optinal reaction component: Coloured reaction buffer, for direct gel loading)
    Optionally supplied, if needed:
  • 25 mM MgCl2 solution. If required, please request this component along with your order.
Additional Resources

 PCR Master Mix Logsheet

Are you looking for a specific product?. Why not browse through our complete catalogue.
(Note: Use * as wildcard)

Quantity / Catalog ID
Add products to your shopping cart by filling in quantity (left field) and catalog number (right field). Next open your shopping cart to review, apply changes to and place your order.

(0) product(s) in shopping cart


Create new account / Request new password

Subscribe to our electronic newsletter for notifications on new products, prices and special offers.
Subscribe Unsubscribe