Figure 1: Taq PCR Master Mix - Package Contents

• Taq PCR Master Mix [2x] (1.5 mM MgCl₂ final conc.)
• PCR grade water
• 10x Color Load (optional dye mix for direct gel loading)

25 mM MgCl₂ solution for fine tuning of Mg++ ion concentration can be included upon customer's request. If required, please ask us to ship this component along with your order.

Characteristics

• All-Purpose Master Mix: 2x PCR Master Mix complete with optimized reaction buffer and high quality dNTPs.
• Additional Package Contents: Plus PCR pure water and plus optional Color Load Buffer for direct post-PCR gel loading, both provided in separate tubes, respectively.
• High Quality: Same high enzyme purity and enzymatic activity as → Taq DNA Polymerase [Cat No. E2500]).
• Strict Quality Control: Same stringent quality control as specified for → Taq DNA Polymerase [Cat No. E2500]).
• Optimized for Advanced Standardization Levels: Minimizes pipetting errors: Warrants high reproducibility for critical PCR assays.
• Continuous Reproducibility: Stable, reproducible high performance for more than 25 freeze-thaw cycles.
• Free of Contaminating DNA: No residual PCR-amplificable DNA present, as tested with many different amplification targets (including bacterial 16S rRNA genes).
• No false positives.
• Careful and Elaborate Purification: High purity and enzymatic activity as a result of thorough but careful and gentle enzyme purification.
• Enzyme Properties: Intrinsic exonuclease activities: 5'-3' present, 3'-5' none. No proofreading activity.
• Cloning: Enables both TA- and blunt end cloning.
• Also available as: → Taq DNA Polymerase (Non - Master Mix) [Cat No. E2500]).

Application Examples

Figure 2: Comparisonal PCR amplification of an 1.1 kb amplicon (human ccr5 gene) with Taq DNA Polymerase (Cat. No. E2500, non-master-mix format)) and ready-to-use Taq PCR Master Mix (Cat. No. 2520). Molecular weight marker MW (Perfect™ 1kb DNA Ladder). Each PCR reaction: 1.25 U Taq DNA Polymerase, 0.2 mM dNTPs; 50 µl reaction volume. Lanes 2-3: EURx Taq DNA Polymerase (non-master-mix format); 4-5: EURx Taq PCR Master Mix (after 25 freeze-thaw cycles, to demonstrate tolerance for repeated freezing and thawing), 6-7: EURx Taq PCR Master Mix (after 25 freeze-thaw cycles) plus optional Color Load (for direct post-PCR gel loading).

Figure 3: PCR amplification with Taq DNA Polymerase (Cat. No. E2500) in a size range between ~1 and 15 kb. Molecular weight markers MW1 (Perfect™ 1kb DNA Ladder), MW2 (Lambda-DNA [GenBank J02459] / Hind III). Lanes 1.1 to 15 kb: PCR amplification reactions (each reaction 1.25 U EURx Taq DNA Polymerase (non-master-mix format), Pol Buffer B, 0.2 mM dNTPs; 50 µl reaction volume).

Additional Resources

 PCR Master Mix Logsheet

 How to trigger Reverse Transcriptase Activity of Taq DNA Polymerase (Contributed Protocol)