Direct Blood PCR Kit
For PCR reactions directly from whole blood without prior DNA extraction or purification. High fidelity - proofreading.
Detailed Product Description
English Version
Deutsche Version
Melting Temperature (Tm) Calculation
Calculate Tm's using the base-stacking method (nearest-neighbor method).Default parameters are: 500 nM primer concentration, 50 mM salt concentration, 1,5 mM Mg2+ concentration.
Source code is freely downloable at biophp.org
Application Example
Figure: Direct blood proofreading PCR amplification with Direct Blood PCR Kit (Cat. No. E0950) in a size range between 0.5 and 4 kb. Amplification started directly from fresh blood samples [5% v/v] without any prior DNA purification step. Molecular weight marker MW (PerfectPlus™ 1 kb DNA Ladder). Lanes 1.1 to 12 kb: PCR amplification reactions. For further reaction parameters see manual.
Direct Blood PCR Kit - Package Contents
PCR Logsheet
Figure: Direct blood proofreading PCR amplification with Direct Blood PCR Kit (Cat. No. E0950) in a size range between 0.5 and 4 kb. Amplification started directly from fresh blood samples [5% v/v] without any prior DNA purification step. Molecular weight marker MW (PerfectPlus™ 1 kb DNA Ladder). Lanes 1.1 to 12 kb: PCR amplification reactions. For further reaction parameters see manual.
Direct Blood PCR Kit - Package Contents
- Direct Blood DNA Polymerase (Proofreading)
- 5x Buffer (Standard reaction buffer containing MgCl2)
- dNTP solution
- 25 mM MgCl2 solution
- 50 mM EDTA solution
- DMSO solution
PCR Logsheet