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Color Perpetual OptiTaq DNA Polymerase


"Hot Start" OptiTaq DNA polymerase (Taq+Pfu), MAB inhibited.
  • Robust, long "HotStart" PCR.
  • Moderate "proofreading".
  • Separate enzyme and 10x buffers.
  • Polymerase supplemented with two tracer dyes for direct gel loading.



Detailed product description
    English version       Deutsche Version  

Quantity Package Cat-No. Price in €
200 uE2730-0156.00
500 uE2730-04130.00
1000 uE2730-02245.00
5000 uE2730-031125.00
200 u / Kit (+ dNTPs)EK2730-0163.00
500 u / Kit (+ dNTPs)EK2730-04147.50
1000 u / Kit (+ dNTPs)EK2730-02280.00
5000 u / Kit (+ dNTPs)EK2730-031300.00




Application Example



Figure: Robust and sensitve: Short, medium and long range HotStart PCR amplifications with Perpetual OptiTaq DNA Polymerase (Cat. No. E2720) in a size range between ~1 and 20 kb. Molecular weight marker M1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 20 kb: PCR amplification reactions (each reaction 1.25 U EURx Perpetual OptiTaq DNA Polymerase, Pol Buffer B, 0.2 mM dNTPs; 50 µl reaction volume).

Notes:

Color Perpetual OptiTaq DNA Polymerase is supplemented with two tracing dyes (yellow and red). These dyes allow direct loading of PCR products to agarose gels. Additionally, these dyes provide an optical control for a complete mixing during reaction setup by visualizing the complete suspension of polymerase in the reaction mix. This may prove useful for less experienced users such as teaching classes and student courses. In this Color edition, these dyes are mixed directly to the polymerase . As a consequence, the Color enzyme edition does not allow the end user to run PCR reactions without addition of dyes.

Note, that uncoloured standard Perpetual OptiTaq DNA Polymerase contains both dyes in a separate coloured buffer, giving you a choice to run both colored and uncolored PCR reactions. Thus, when looking for a 10x buffer / optional gel loading dye combination, experienced researchers will want to use Standard Perpetual OptiTaq DNA Polymerase . Among colourless buffers, the standard enzyme package contains a purple coloured buffer Pol C. Purple buffer Pol C allows to load aliquots of PCR reactions directly on gel without the need to add a separate gel loading buffer.

Click here for a direct link to the Perpetual OptiTaq DNA Polymerase product page.

Additional Resources

 PCR Logsheet



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